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KMID : 1171820090240010040
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Journal of Environmental and Sanitary Engineering
2009 Volume.24 No. 1 p.40 ~ p.46
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Alkaline α-amylaseProduction from Bacillus megaterium
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Jia Shi Ru
Lim Chae-Kyu
Seo Gwang-Yeob
Nam Hyung-Gun
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Abstract
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The enzyme expressed from strain L-49 was 2.01 times higher than that of original strain. Strain L-49 can grow on culture plate with 50㎍/mL ampicillin. The synthesis of α-amylase was greatly suppressed when strain L-49 was grown on monosaccharide such as glucose and polysaccharide at the same time cell concentration was low. Amylase production was enhanced when the bacterium was grown on starch and dextrin. Among different nitrogen sources tried, yeast extract was found to be the best followed by panpeptone, peptone, meat extract, bean meal, and corn steep liquor. The average rate of enzyme production was enhanced for 3~4 times in fermentation time from 24h to 44h.. The sugar uptake rate has also increased. Low oxygen supply rate enhanced the rate of strain propagation but depressed the enzyme production. Hence it is benefit to obtain high enzyme activity that agitation speed maintained not lower than 400r/min and aeration rate maintained greater than 1:1vvm.
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